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Chapitres
- 00:04Titre
- 00:56Preparations
- 01:42Extraction of the Small Intestines from the Abdominal Cavity
- 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
- 04:58Completing the Procedure and Collecting Results
- 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
- 08:02Conclusion
Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.
