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En sammenskrevne Intestinal Loop Model i bedøvede specifikke patogen gratis kyllinger at studere Clostridium Perfringens virulens
Journal JoVE
Médecine
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Journal JoVE Médecine
A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
DOI:

09:21 min

October 11, 2018

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Chapitres

  • 00:04Titre
  • 00:56Preparations
  • 01:42Extraction of the Small Intestines from the Abdominal Cavity
  • 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
  • 04:58Completing the Procedure and Collecting Results
  • 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
  • 08:02Conclusion

Summary

Traduction automatique

Her præsenterer vi en protokol for at kirurgisk oprette intestinal sammenskrevne sløjfer i kylling små tarme. Denne procedure giver mulighed for sammenligning af flere Clostridium perfringens stammer virulens i situ i en enkelt vært. Denne metode reducerer markant antallet af kyllinger normalt nødvendigt for lignende i vivo eksperimenter.

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