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小鼠初级少突胶质的快速特异磁分离

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

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小鼠初级少突胶质的快速特异磁分离

DOI:

10.3791/57543-v

•

09:32 min

•

May 21, 2018

•

Rafael E. Flores-Obando, Mona M. Freidin, Charles K. Abrams

¹Program in Molecular and Cellular Biology,State University of New York Downstate Medical Center, ²Department of Neurology and Rehabilitation,University of Illinois at Chicago

Chapitres

00:05Titre
00:40Mouse Brain Cortex Dissociation
04:49O4+ Oligodendrocyte Isolation and Plating
07:26Results: Representative Cell Surface Marker Expression During Mouse Oligodendrocyte Development
09:05Conclusion

Summary

Traduction automatique

我们描述了主鼠标少突胶质的磁隔离, 它允许对体外区域性的细胞进行快速和特定的隔离。

Tags

Immunomagnetic Isolation Mouse Primary Oligodendrocytes Myelin Myelination Neural Science Cell Culture Dissociation Neurobasal A Medium Bovine Growth Serum DNase I Pipetting

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