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Drosophila i Vivo skade modell for å studere Neuroregeneration i tilleggsutstyret og sentralnervesystemet
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Médecine
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Journal JoVE Médecine
A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

Drosophila i Vivo skade modell for å studere Neuroregeneration i tilleggsutstyret og sentralnervesystemet

DOI:
10.3791/57557-v

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09:55 min

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May 05, 2018

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, , ,
1Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine,University of Pennsylvania

Chapitres

  • 00:04Titre
  • 01:08Collecting Larvae
  • 02:11Microscope Set Ups
  • 04:18Two-photon Injury
  • 07:40Results: Axon Regeneration in the PNS
  • 08:49Conclusion

Summary

Traduction automatique

Traduction automatique

Her presenterer vi en protokoll med Drosophila sensoriske Nevron - dendrittiske arborization (da) Nevron skade modellen, som kombinerer i vivo live bildebehandling, to-fotonet laser axotomy/dendriotomy og kraftig fly genetisk verktøykassen, som en plattform for screening potensielle arrangører og hemmere av neuroregeneration.

Tags

DrosophilaIn Vivo Injury ModelNeuroregenerationPeripheral Nervous SystemCentral Nervous SystemLive ImagingTwo-photon Laser Axotomy/dendriotomyGenetic ToolboxScreeningAxon RegenerationDendritic RegenerationNeurodegenerative DiseasesNeuron-glia InteractionsLarval CollectionCulture BottlesGrape Juice Agar PlateImagingTwo-photon MicroscopeGFPLaser IntensityPNS InjuryVNC Injury

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