Journal
/
Médecine
/
Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

DOI:
10.3791/57560-v

•

09:00 min

•

August 02, 2018

•

, , ,
1Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program,Ohio State University Wexner Medical Center

Chapitres

  • 00:04Titre
  • 01:23Design and Cloning of gRNAs into the CRISPR Vector
  • 05:51Intraperitoneal Injection of rAAVrh.74-CRISP into Neonatal mdx Mice
  • 06:38Analysis of Dystrophin Expression by Immunofluorescence Staining
  • 07:31Results: rAAV-Mediated Gene Editing Restores Dystrophin Expression in Dystrophic Mice
  • 08:34Conclusion

Summary

Traduction automatique

Traduction automatique

Here we provide a detailed protocol to carry out in vivo cardiac gene editing in mice using recombinant Adeno-Associated Virus(rAAV)-mediated delivery of CRISPR. This protocol offers a promising therapeutic strategy to treat dystrophic cardiomyopathy in Duchenne muscular dystrophy and can be used to generate cardiac-specific knockout in postnatal mice.

Tags

Keywords: Adeno-associated VirusCRISPRCardiac Gene EditingMuscular DystrophyDystrophinDuchenne’s Muscular DystrophyGene EditingGene TherapyGRNA DesignCRISPR VectorLigation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57560?article_type=v

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
check_url/fr/57560?article_type=v

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
check_url/fr/57560?article_type=v

Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice
check_url/fr/57560?article_type=v

An Orthotopic Bladder Cancer Model for Gene Delivery Studies
check_url/fr/57560?article_type=v

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
check_url/fr/57560?article_type=v

Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease
check_url/fr/57560?article_type=v

Development of an Alpha-synuclein Based Rat Model for Parkinson’s Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector
check_url/fr/57560?article_type=v

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
check_url/fr/57560?article_type=v

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
check_url/fr/57560?article_type=v

Intratracheal Administration of Dry Powder Formulation in Mice

Read Article