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An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm
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Journal JoVE Biologie
An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

DOI:
10.3791/57604-v

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05:12 min

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April 16, 2018

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, , , , , , ,
1Department of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, Shanghai Key Clinical Disciplines of otorhinolaryngology,Fudan University, 2Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Department of Medical Microbiology and Parasitology, School of Basic Medical Science,Shanghai Medical College of Fudan University, 3Department of Dermatology, Huashan Hospital,Fudan University

Chapitres

  • 00:04Titre
  • 00:40Biofilm Formation and Light Irradiation
  • 02:17Photodynamic Therapy Analysis
  • 04:00Results: Representative Biofilm Viability Analyses
  • 04:33Conclusion

Summary

Traduction automatique

Traduction automatique

This manuscript describes a protocol to study the antimicrobial effect of 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) on a Staphylococcus aureus biofilm. This protocol can be used to develop an in vitro model to study the treatment of bacterial biofilms with PDT in the future.

Tags

Keywords: 5-aminolevulinic AcidPhotodynamic TherapyStaphylococcus AureusBiofilmIn Vitro ModelAnti-microbial EffectBacterial InfectionCell CultureLight-based TreatmentViable Bacteria Count

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