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1Department of Internal Medicine-Gastroenterology,University of Michigan, 2Department of Molecular and Integrative Physiology,University of Michigan, 3Department of Molecular, Cellular and Developmental Biology,University of Michigan, 4Department of Gastrointestinal Surgery,The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology,University of Arizona College of Medicine
Chapitres
- 00:04Titre
- 00:33Dissection of the Intestinal Segment
- 03:01Removal of the Epithelial Mucosa
- 04:57Collection of Enteric Glial Cells from the Lamina Propria and Submucosa
- 06:23Results: Enteric Glial Cells 24 h After Plating
- 07:12Conclusion
Her beskriver vi isolering af entero-glial celler fra den tarm-submucosa bruge sekventielle EDTA inkubationer til Chelat divalent kationer og derefter inkubation i ikke-enzymatiske celle opsving løsning. Plating den resulterende cellesuspension på poly-D-Lysin og laminin resulterer i en yderst højberiget kultur af submukøse glial celler for funktionelle analyse.
