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1Department of Orthopaedic Surgery, CHA Bundang Medical Center,CHA University School of Medicine, 2Department of Pathology, Kyung Hee University Hospital at Gangdong,Kyung Hee University School of Medicine, 3Department of Physiology,CHA University School of Medicine, 4Department of Rehabilitation, CHA Bundang Medical Center,CHA University School of Medicine
Chapitres
- 00:04Titre
- 01:01Tendon Tissue Sample Extraction
- 02:39Tenocyte Culture and Immunocytochemistry (ICC)
- 05:10Hematoxylin and Eosin (H&E) and Immunohistochemical (IHC) Analysis
- 07:36Results: Representative Characteristics of Degenerative Tenocytes
- 08:59Conclusion
In vitro use of degenerative tenocytes is essential when investigating the efficacy of novel treatment on tendinopathy. However, most research studies use only the animal model or a healthy tenocyte. We propose the following protocol to isolate human degenerative tenocytes during surgery.
