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Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

DOI:
10.3791/57648-v

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08:44 min

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June 05, 2018

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,
1College of Nursing,University of Arizona

Chapitres

  • 00:04Titre
  • 00:51Skeleton Analysis
  • 05:10Fractal Analysis
  • 07:19Results: Comparison of Data Analysis with and without Application of the Protocol
  • 08:10Conclusion

Summary

Traduction automatique

Traduction automatique

Microglia are brain immune cells that survey and react to altered brain physiology through morphologic changes which may be evaluated quantitatively. This protocol outlines an ImageJ based analysis protocol to represent microglia morphology as continuous data according to metrics such as cell ramification, complexity, and shape.

Tags

Keywords: Microglia MorphologyImmunohistochemistryImageJSkeleton AnalysisFractal AnalysisNeuroinflammmatoryContinuous VariablesIba1FFT Bandpass FilterUnsharp MaskDespeckleBinarySkeletonize

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