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Lys arks mikroskopi for tredimensjonale visualisering av menneskelig immunceller
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
DOI:

09:44 min

June 13, 2018

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Chapitres

  • 00:04Titre
  • 00:53Preparation of Neutralized Collagen
  • 01:55Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
  • 04:08Image Acquisition Using Light-sheet Microscopy
  • 05:07Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
  • 07:17Results: Visualization of Human CTL Cells by Light-sheet Microscopy
  • 08:32Conclusion

Summary

Traduction automatique

Her presenterer vi en protokoll for å visualisere immunceller i en tredimensjonal (3D) kollagen matrise med lys arks mikroskopi. Denne protokollen også utdyper hvordan spore celle migrasjon i 3D. Denne protokollen kan benyttes for andre typer suspensjon celler i 3D matrix.

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