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Fabrikation af Gradient Nanopattern af termisk Nanoimprinting teknik og Screening af svar i Endothelial kolonidannende celler
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells
DOI:

11:24 min

July 01, 2018

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Chapitres

  • 00:04Titre
  • 00:36Preparation of Aluminum (Al) Substrate by Electropolishing
  • 01:34Fabrication of Gradient Anodic Aluminum Oxide (AAO) Mold with Phosphoric Acid Electrolyte
  • 04:28Deposition of Anti-Sticking Layer on Gradient AAO Mold with Self-Assembled Monolayer
  • 05:46Fabrication of Gradient Nanopattern Plates by Thermal Imprinting
  • 07:08Cultivation of hECFCs and Culture on the Gradient Nanopattern Plates
  • 09:14Results: Evaluation and Analysis of Culturing hECFCs on Fabricated Gradient Nanopattern Plates
  • 10:37Conclusion

Summary

Traduction automatique

Vi præsenterer her, en protokol for fabrikation af gradient nanopattern plader via termisk nanoimprinting og metoden til screening svar af menneskelige endotelceller stamceller til nanostrukturer. Ved hjælp af den beskrevne teknologi, er det muligt at producere et stillads, der kan manipuleres celle adfærd ved fysiske stimuli.

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