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Fabrikasjon av Gradient Nanopattern av termisk Nanoimprinting teknikk og Screening av responsen av menneskelig Colony-forming endotelceller
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells
DOI:

11:24 min

July 01, 2018

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Chapitres

  • 00:04Titre
  • 00:36Preparation of Aluminum (Al) Substrate by Electropolishing
  • 01:34Fabrication of Gradient Anodic Aluminum Oxide (AAO) Mold with Phosphoric Acid Electrolyte
  • 04:28Deposition of Anti-Sticking Layer on Gradient AAO Mold with Self-Assembled Monolayer
  • 05:46Fabrication of Gradient Nanopattern Plates by Thermal Imprinting
  • 07:08Cultivation of hECFCs and Culture on the Gradient Nanopattern Plates
  • 09:14Results: Evaluation and Analysis of Culturing hECFCs on Fabricated Gradient Nanopattern Plates
  • 10:37Conclusion

Summary

Traduction automatique

Her presenterer vi en protokoll for fabrikasjon av gradient nanopattern plater via termisk nanoimprinting og metode for screening svar av menneskelig endoteliale progenitor celler å nanostrukturer. Bruker den beskrevet teknologien, er det mulig å produsere et stillas som kan manipulere celle oppførsel ved fysisk stimuli.

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