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Immunologie et infection

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印制糖类阵列是分析小动物循环抗碳水化合物抗体汇辑的一种敏感技术

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Immunologie et infection

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Journal JoVE Immunologie et infection

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

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印制糖类阵列是分析小动物循环抗碳水化合物抗体汇辑的一种敏感技术

DOI:

10.3791/57662-v

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08:49 min

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February 14, 2019

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Sara Olivera-Ardid*¹, Nailya Khasbiullina*^2,3, Alexey Nokel, Andrey Formanovsky, Inna Popova, Tatiana Tyrtysh, Roman Kunetskiy, Nadezhda Shilova, Nicolai Bovin⁴, Daniel Bello-Gil, Rafael Mañez⁵

¹Infectious Pathology and Transplantation Division,Bellvitge Biomedical Research Institute (IDIBELL), ²Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh),Russian Academy of Sciences, ³Semiotik LLC, ⁴Auckland University of Technology, ⁵Intensive Care Department,Bellvitge University Hospital

Chapitres

00:04Titre
00:41Glycochips Production
01:39Glycan Array Technique
04:44Analysis of Glycan Array
06:09Results: Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in BALB/c Mice
07:26Conclusion

Summary

Traduction automatique

这项工作显示了印刷糖类阵列 (pga) 技术在小动物循环抗碳水化合物抗体分析方面的潜力。

Tags

Glycan Array Anti-carbohydrate Antibodies Small Animals Serum Printed Glycan Array Glycan Targets Polysaccharides PBS N-hydroxy 6 Cinnamic Derivatized Glass Slides Blocking Buffer Buffer Mice Serum Immunoglobulin Aggregation Centrifugation Incubation Chamber

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