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促纤维化信号增强因子介导的小鼠胚胎成纤维细胞再编程诱导心肌细胞的抑制

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Biologie du développement

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Journal JoVE Biologie du développement

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

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促纤维化信号增强因子介导的小鼠胚胎成纤维细胞再编程诱导心肌细胞的抑制

DOI:

10.3791/57687-v

•

09:16 min

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June 03, 2018

•

Andrew S. Riching, Yuanbiao Zhao, Yingqiong Cao, Pilar Londono, Hongyan Xu, Kunhua Song

¹Division of Cardiology, Department of Medicine,University of Colorado Anschutz Medical Campus, ²Department of Population Health Sciences, Medical College of Georgia,Augusta University

Chapitres

00:05Titre
00:46Production of Retrovirus and Transduction of MEFs
05:01Immunostaining of Reprogrammed MEFs
06:44Results: Over-expression of GHMT2m and Inhibition of TGF-β Signaling Increases the Reprogramming Efficiency of MEFs into Functional Cardiomyocytes
08:34Conclusion

Summary

Traduction automatique

通过 GATA4、Hand2、Mef2c、Tbx5、miR-1 和 miR-133 (GHMT2m) 的过度表达和 TGF β信号的抑制, 我们提出了一种健壮的方法, 将原发成纤维细胞重新编程成功能性心肌细胞。我们的协议在7天后的转导中产生跳动的心肌细胞, 效率高达60%。

Tags

Pro-fibrotic Signaling Factor-mediated Reprogramming Induced Cardiomyocytes Cardiac Reprogramming Fibroblast To Cardiomyocyte Conversion Cardiomyocyte Generation Chemic Heart Disease Fibroblast Transfection Retrovirus Production Platinum E Cells GHMT2M Cocktail Mouse Embryonic Fibroblasts Collagen Coating

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