Journal
/
/
Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
DOI:

05:27 min

August 08, 2018

,

Chapitres

  • 00:04Titre
  • 00:23HaCaT Monolayer Generation
  • 01:50Migration Stimulation and Optical Camera Setup
  • 03:59Results: Representative Wound Healing Analyses
  • 04:54Conclusion

Summary

Traduction automatique

Here we describe a procedure to test the cell migration in vitro with an automated optical camera microscope. The scratch assay has been widely used where the closure of the scratch is followed for a set period. The optical microscope enables dependable and cheap detection of cell migration.

Vidéos Connexes

Read Article