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A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
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Neurosciences
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Journal JoVE Neurosciences
A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

DOI:
10.3791/57698-v

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07:13 min

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May 07, 2018

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, , ,
1Department of Pharmacology, College of Medicine,University of Arizona, Tucson

Chapitres

  • 00:04Titre
  • 00:54Extraction and Processing of Brain Tissue from Sprague-Dawley Rats
  • 02:59Centrifugation
  • 04:27Results: Analysis of Freshly Isolated Rat Brain Microvessel Samples
  • 06:07Conclusion

Summary

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Here, a method for isolation of rat brain microvessels and for the preparation of membrane samples is described. This protocol has the clear advantage of producing enriched microvessel samples with acceptable protein yield from individual animals. Samples can then be used for robust protein analyses at the brain microvascular endothelium.

Tags

Brain MicrovesselsBlood-brain BarrierRat BrainCerebral CortexProtease InhibitorBiochemical StudiesMembrane Isolation

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