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Split grøn fluorescerende proteiner System til at visualisere effektorer leveret fra bakterier under infektion
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Journal JoVE Biologie
Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Split grøn fluorescerende proteiner System til at visualisere effektorer leveret fra bakterier under infektion

DOI:
10.3791/57719-v

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07:25 min

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May 24, 2018

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, , , ,
1Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science,Seoul National University, 2Department of Bioindustry and Bioresource Engineering,Sejong University

Chapitres

  • 00:04Titre
  • 00:49Preparation of Plant Materials and Pseudomonas Culture
  • 02:09Transient Expression of Organelle-targeted sfGFP1-10OPT de N. benthamiana
  • 03:58Inoculation of Pseudomonas and Observation of sfGFP Signal via Confocal Microscopy
  • 05:46Results: Split Superfolder Fluorescent Protein System: A Useful Tool to Examine the Dynamics of Effectors in Host Plants
  • 06:32Conclusion

Summary

Traduction automatique

Traduction automatique

Fluorescerende protein-baserede tilgange til at overvåge effektorer udskilles af bakterier i værtsceller er udfordrende. Dette er på grund af inkompatibilitet mellem fluorescerende proteiner og type III sekretion system. Her, anvendes en optimeret split superfolder normal god landbrugspraksis system til visualisering af effektorer udskilles af bakterier i vært plante celle.

Tags

Split Green Fluorescent ProteinEffectorsPlant-microbe InteractionType III Secretion SystemPseudomonas SyringaeAgrobacterium TumefaciensInfiltrationVisualization

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