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Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

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Génétique

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Journal JoVE Génétique

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

DOI:

10.3791/57731-v

•

09:30 min

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September 13, 2018

•

Clark Fritsch², Jean-Francois Pierre Gout⁴, Marc Vermulst

¹Center for Mitochondrial and Epigenomic Medicine,Children’s Hospital of Philadelphia, ²Department of Cellular and Molecular Biology,University of Pennsylvania, ³Department of Biological Sciences,Mississippi State University, ⁴Center for Mechanisms of Evolution, Biodesign Institute,Arizona State University

Chapitres

00:04Titre
00:38RNA Circularization and Rolling Circle Reverse Transcription
02:51Second Strand cDNA Synthesis and Repair
03:45Adapter Ligation and Size Selection
06:21PCR Amplification and Final Bead Purification
07:53Results: Quality Control, Bio-Informatic Analysis, and Expected Results from the C-seq Assay
08:54Conclusion

Summary

Traduction automatique

This protocol provides researchers with a new tool to monitor the fidelity of transcription in multiple model organisms.

Tags

Transcription Errors Eukaryotic Organisms Transcriptional Mutagenesis RNA Polymerase Bioinformatics Circularize RNA T4 RNA Ligase Reverse Transcription Second Strand Synthesis Genome-wide Surveillance

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