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Netthinnen Cryo-deler, hele-monteringer, og hypotonisk isolert blodkar forberedelser for Immunohistochemical visualisering av mikrovaskulær Pericytes
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Biologie
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Journal JoVE Biologie
Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Netthinnen Cryo-deler, hele-monteringer, og hypotonisk isolert blodkar forberedelser for Immunohistochemical visualisering av mikrovaskulær Pericytes

DOI:
10.3791/57733-v

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10:46 min

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October 07, 2018

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, ,
1Department of Clinical Experimental Research,Glostrup Research Institute, 2Department of Clinical Sciences, Division of Experimental Vascular Research,Lund University

Chapitres

  • 00:04Titre
  • 00:41Cryosection Rat Retinal Tissue Preparation and Immunostaining
  • 03:23Whole Mount Rat Retinal Tissue Preparation and Immunostaining
  • 05:08Hypotonic Isolation of Ray Retinal Tissue Preparation and Immunostaining
  • 08:27Results: Representative Immunohistochemistry Images
  • 10:18Conclusion

Summary

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Traduction automatique

Viser vi tre ulike vev forberedelser teknikker for immunohistochemical visualisering av rotte retinal mikrovaskulær pericytes, dvs. cryo-partiene, hele-montere og hypotonisk isolasjon av vaskulære nettverket.

Tags

Retinal Cryo-sectionsWhole-mountsIsolated VasculatureImmunohistochemistryPericytesEnucleationFormaldehyde FixationCryoprotectionCryosectioningAntibody StainingFluorescent LabelingMicroscopy

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