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Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
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Journal JoVE Génétique
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

DOI:
10.3791/57737-v

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08:27 min

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May 21, 2018

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, ,
1Department of Veterans Affairs,Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine,Emory University

Chapitres

  • 00:04Titre
  • 01:01Mouse Acute Septic Lung Injury
  • 02:05Exosome Isolation and Purification
  • 03:41Exosome Recipient Uptake and Interaction Validation
  • 05:30Results: BALF-isolated Exosome Characterization
  • 06:53Conclusion

Summary

Traduction automatique

Traduction automatique

Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.

Tags

Bronchoalveolar LavageExosomesLipopolysaccharideSeptic Lung InjuryAcute Lung InjuryAcute Respiratory Distress SyndromeAlveolar MicroenvironmentExosome IsolationCentrifugationUltracentrifugationSyringe FiltrationExosome Characterization

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