Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Ruby Cheung; Gregory W. deHart; Lynne Jesaitis; Stephen J. Zoog; Andrew C. Melton

doi:10.3791/57777

Journal

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Immunologie et infection

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Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

DOI:

10.3791/57777-v

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07:52 min

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September 10, 2018

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Ruby Cheung, Gregory W. deHart, Lynne Jesaitis, Stephen J. Zoog, Andrew C. Melton

¹BioMarin Pharmaceutical Inc

Chapitres

00:04Titre
00:56Cell Plating and Sample Preparation
03:43Sample Addition and Cell Viability Staining
05:44Results: Representative Neutralizing Antibody (NAb) Detection by Enzyme Replacement Therapy (ERT)
07:09Conclusion

Summary

Traduction automatique

Here we present a cell-based flow cytometry method to detect neutralizing antibodies or other factors that interfere with the cellular uptake of enzyme replacement therapies in a human matrix, such as cerebral spinal fluid (CSF) or human serum.

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

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