Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:04Titre
- 00:56Cloning the Recipient Vector to Include a Tissue-specific Promoter
- 03:48Cloning the Sponge
- 04:55Generation of Stable H9c2 miR-181-sponge Expressing Cells
- 05:53Synthesis and Purification of Sponge-nanoparticles (miR-181-sponge Nanoparticles)
- 07:01Results: Validation of Nanonvector Delivery of the miR-181-sponge and its Effect on miR-181c Expression
- 07:58Conclusion
Væv-specifikke microRNA hæmning er en teknologi, der er underudviklede i feltet microRNA. Heri, beskriver vi en protokol for at kunne hæmme familien miR-181 microRNA i myoblast celler fra hjertet. Nanovector teknologi bruges til at levere en mikroRNA svamp, der viser betydelig i vivo cardio-specifikke miR-181 familie hæmning.
