微粒相关α synuclein 聚合体对原代神经元的外源性管理--纤维形成的强大细胞模型

Journal JoVE
Neurosciences

This content is Free Access.

Journal JoVE Neurosciences

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

微粒相关α synuclein 聚合体对原代神经元的外源性管理--纤维形成的强大细胞模型

DOI:

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09:16 min

June 26, 2018

00:04Titre
00:36Isolation of Microsomes-associated Alpha-synuclein Aggregates from Diseased A53T Alpha-synuclein Transgenic Mic
02:30Western Blot
04:32Neurons Treatment
05:40Immunofluorescence
07:45Results: Time-dependent Induction of Alpha-synuclein Deposition After Administration of Microsomes-associated Alpha-synuclein Aggregates
08:35Conclusion

本协议的目标是提供一个基于细胞的系统,在体内复制α synuclein 聚合体的形成。细胞内阿尔法-synuclein 包裹体的种子在原代神经元的内化和传播的外源管理的原生微粒相关的阿尔法-synuclein 集分离的病阿尔法 synuclein 转基因小鼠。