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基因型衰老过程中正常人成纤维细胞活性氧和衰老相关分泌表型的定量测定
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
DOI:

13:59 min

August 12, 2018

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Chapitres

  • 00:00Titre
  • 01:10Inducing Oncogene-induced Senescence
  • 03:11Monitoring Senescence by Senescence Associated ß-galactosidase (SA ß-gal) Staining
  • 04:27Quantifying ROS Induction during OIS
  • 06:42Quantifying IL-6 and IL-8 mRNA Expression for SASP Anaylsis using Real-time PCR
  • 08:53Quantifying the Levels of Secreted IL-6 and IL-8 Proteins for SASP Analysis using ELISA
  • 10:56Results: Representative ROS and SASP Expression Analysis during H-Ras-induced Senescence
  • 12:25Conclusion

Summary

Traduction automatique

胞内活性氧在细胞衰老诱导中起着重要作用。在这里, 我们描述了一个敏感的测定细胞衰老过程中的 ROS 水平。我们还提供了评估衰老相关分泌表型的协议, 据报告, 这有助于各种年龄相关的功能障碍。

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