Journal
/
Biologie
/
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies
Journal JoVE
Biologie
Author Produced
This content is Free Access.
Journal JoVE Biologie
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

DOI:
10.3791/57944-v

•

12:42 min

•

July 14, 2018

•

, , , , , , , ,
1Centre for Experimental Medicine,Queen’s University of Belfast, 2Centre for Biomedical Sciences (Education),Queen’s University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy,Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences,Queen’s University of Belfast

Chapitres

  • 00:00Titre
  • 01:23Isolation of Retinal Arterioles
  • 05:09Arteriolar Pressure Myography
  • 07:22Patch-Clamp Electrophysiology
  • 09:58Résultats
  • 12:00Conclusion

Summary

Traduction automatique

Traduction automatique

This manuscript describes a straightforward protocol for the isolation of arterioles from the rat retina that can be used in electrophysiological, calcium imaging and pressure myography studies.

Tags

Retinal ArteriolesRetinal Blood FlowRetinal PerfusionVascular Smooth MusclePatch ClampCalcium ImagingPressure MyographyRetinal DiseasesDiabetic RetinopathyGlaucomaBranch Vein OcclusionsEye DissectionRetina IsolationLow Calcium Hanks’ Solution

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57944?article_type=v

Mouse Adrenal Chromaffin Cell Isolation
check_url/fr/57944?article_type=v

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
check_url/fr/57944?article_type=v

In vivo-like Organotypic Murine Retinal Wholemount Culture
check_url/fr/57944?article_type=v

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
check_url/fr/57944?article_type=v

The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity
check_url/fr/57944?article_type=v

An Ex vivo Culture System to Study Thyroid Development
check_url/fr/57944?article_type=v

Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines
check_url/fr/57944?article_type=v

Ex Vivo Method for Assessing the Mouse Reproductive Tract Spontaneous Motility and a MATLAB-based Uterus Motion Tracking Algorithm for Data Analysis
check_url/fr/57944?article_type=v

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
check_url/fr/57944?article_type=v

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Read Article