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Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells

Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells

DOI:
10.3791/57995-v

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05:43 min

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July 27, 2018

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, , ,
1MRC Centre for Regenerative Medicine,University of Edinburgh

Chapitres

  • 00:04Titre
  • 00:30Seeding Human Pluripotent Stem Cells (hPSCs)
  • 02:32Differentiating hPSCs on Recombinant Laminins and Characterization
  • 04:01Results: Verification of Successful Production via Hepatocyte Markers
  • 04:58Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a semi-automated approach to produce hepatocyte-like cells from human pluripotent stem cells in a 96 well plate format. This process is rapid and cost-effective, allowing the production of quality assured batches of hepatocyte-like cells for basic and applied human research.

Tags

Semi-automatedHepatocyte-like CellsPluripotent Stem CellsLaminin 521MTeSR1 MediumROCK Inhibitor Y27632Definitive Endoderm InductionKSR DMSO MediumHepatoblast SpecificationHepatoZYME Medium96-well FormatAutomated Liquid HandlingMass ProductionCell ConfluencyDifferentiation

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