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Biologia celular quantitativa de neurodegeneração em Drosophila , através de uma análise imparcial das proteínas fluorescente etiquetadas usando o ImageJ
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1Department of Molecular and Cellular Pharmacology,University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Yantai University
Chapitres
- 00:04Titre
- 00:50Brain Dissection and Immunofluorescence Staining
- 02:23Image Acquisition and Region of Interest (ROI) Selection
- 04:19Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification
- 06:30Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration
- 08:20Conclusion
Nós desenvolvemos um fluxo de trabalho simples e adaptável para extrair dados quantitativos de estudos biológicos fluorescência-imagem latente-à base de célula de agregação de proteínas e autophagic fluxo no sistema nervoso central de Drosophila modelos de Neurodegeneração.
Tags
Quantitative Cell BiologyNeurodegenerationDrosophilaFluorescently Tagged ProteinsImageJImage Analysis WorkflowProteinaceous PunctaMembrane-bound CompartmentsNeurodegenerative DiseasesLamina DissectionRetina RemovalBrain DissectionImmunostainingAntibody IncubationMountingMicroscopyFluorescence Imaging
