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Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
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Génétique
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Journal JoVE Génétique
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

DOI:
10.3791/58044-v

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07:20 min

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August 31, 2018

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, ,
1Biological Sciences Department,Vanderbilt University

Chapitres

  • 00:04Titre
  • 00:34Plant and Aphid Culture
  • 02:24DNA Quantification
  • 04:19RNA Sequencing Preparation
  • 06:01Results: Measuring Gene Expression in Aphis nerd
  • 06:50Conclusion

Summary

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The aphid Aphis nerii colonizes on highly-defended plants in the dogbane family (Apocyanaceae) and provides numerous opportunities to study plant-insect interactions. Here, we present a series of protocols for the maintenance of plant and aphid cultures, and the generation and analysis of molecular and -omic data for A. nerii.

Tags

Aphid CulturingGene ExpressionAphis NeriiPlant-insect InteractionsNon-model SystemSeedling TrayPotting SoilMouth PipetteIso-clonal LineCup CageControlled EnvironmentDNA ExtractionLysis Buffer

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