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Immunologie et infection

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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离

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Immunologie et infection

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Journal JoVE Immunologie et infection

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离

DOI:

10.3791/58061-v

•

07:21 min

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July 29, 2018

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Alberto Yáñez², Helen S. Goodridge²

¹Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, ²Research Division of Immunology,Cedars-Sinai Medical Center

Chapitres

00:04Titre
00:32Magnetic-activated Cell Sorting (MACS) for Progenitor Enrichment
02:06Fluorescence-activated Cell Sorting (FACS) for Myeloid Progenitor Identification and Isolation
06:07Results: Representative Progenitor Yields and Purities
06:45Conclusion

Summary

Traduction automatique

我们展示了如何用磁性和荧光分选 (mac 和流动力学) 相结合的方法, 从小鼠骨髓中识别和分离出6个髓样祖细胞。本协议可用于体外培养测定 (纤维素或液体培养),体内移植实验, RNA/蛋白质分析。

Tags

Myeloid Progenitors Bone Marrow MACS Lineage-negative Cells FACS Neutrophils Monocytes Dendritic Cells Cell Isolation Hematology Immunology

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