Journal
/
Immunologie et infection
/
A High-throughput, High-content, Liquid-based C. elegans Pathosystem
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
A High-throughput, High-content, Liquid-based C. elegans Pathosystem

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

DOI:
10.3791/58068-v

•

09:44 min

•

July 01, 2018

•

, ,
1Department of Biosciences,Rice University

Chapitres

  • 00:04Titre
  • 00:25Preparation and Maintenance of P. aeruginosa and RNAi Bacteria
  • 02:34Maintenance and Preparation of C. elegans
  • 03:21Liquid Killing Assay Setup
  • 05:56Adaptation for Screening Multiple C. elegans Strains or Knockdowns
  • 07:06Results: C. elegans-P. aeruginosa Liquid Killing Pathosystem
  • 08:37Conclusion

Summary

Traduction automatique

Traduction automatique

Here we describe a protocol that is an adaptable, whole host, high-content screening tool that can be utilized to study host-pathogen interactions and be used for drug discovery.

Tags

High-throughputHigh-contentLiquid-basedC. ElegansPathosystemHost-pathogen InteractionDrug DiscoveryVirulence FactorsSmall MoleculesP. AeruginosaLB AgarLB BrothSlow Kill (SK) MediaRNAiCarbenicillinIPTGL1 WormsOP50Temperature-sensitiveCell Scraper

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/58068?article_type=v

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
check_url/fr/58068?article_type=v

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
check_url/fr/58068?article_type=v

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
check_url/fr/58068?article_type=v

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
check_url/fr/58068?article_type=v

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
check_url/fr/58068?article_type=v

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
check_url/fr/58068?article_type=v

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
check_url/fr/58068?article_type=v

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
check_url/fr/58068?article_type=v

Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform
check_url/fr/58068?article_type=v

A High-throughput Shigella-specific Bactericidal Assay

Read Article