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Karakterisering av MLKL-mediert Plasma membranen brudd i Necroptosis
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Biologie
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Journal JoVE Biologie
Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Karakterisering av MLKL-mediert Plasma membranen brudd i Necroptosis

DOI:
10.3791/58088-v

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08:55 min

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August 07, 2018

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, , , ,
1Department of Structural Biology,St. Jude Children’s Research Hospital, 2Department of Chemical Biology and Therapeutics,St. Jude Children’s Research Hospital, 3Department of Immunology,St. Jude Children’s Research Hospital

Chapitres

  • 00:04Titre
  • 00:50Mixed Lineage Kinase Domain-like (MLKL)-mediated Necroptosis Live-cell Microscopy Imaging
  • 03:26Nuclear Magnetic Resonance (NMR) Spectroscopy MLKL Lipid Binding
  • 05:35Results: Representative MLKL: Plasma Membrane Interaction Analyses
  • 07:26Conclusion

Summary

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Vi rapporterer metoder for kategorisering av MLKL-mediert plasma membranen brudd i necroptosis inkludert konvensjonelle og AC confocal live-celle mikroskopi bildebehandling, skanning elektronmikroskop og NMR-baserte lipid binding.

Tags

Keywords: MLKLNecroptosisPlasma Membrane RuptureLive-cell ImagingNuclear Magnetic ResonanceLipid BindingMembrane TranslocationPermeabilizationMouse Embryonic FibroblastsDoxycyclineFK506-binding Protein DimerizerFluorescence MicroscopyCell Death Quantification

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