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Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

DOI:
10.3791/58109-v

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10:38 min

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September 21, 2018

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, , , , ,
1Department of Cell Biology, Sciences III,University of Geneva

Chapitres

  • 00:04Titre
  • 00:46Purification of C. reinhardtii Centrioles
  • 05:15Concentration of Centrioles onto the Center of the Coverslips
  • 07:06Quantification of Isolated Centrioles on Coverslips: Centrifugation and Immunofluorescenc
  • 08:47Results: Isolation and Fluorescence Imaging of Chlamydomonas Centrioles
  • 10:03Conclusion

Summary

Traduction automatique

Traduction automatique

We have developed a strategy to purify and image a large number of centrioles in different orientations amenable for super-resolution microscopy and single-particle averaging.

Tags

Keywords: CentrioleChlamydomonasSingle-particle ReconstructionFluorescence ImagingIsolationProtein LocalizationDeflagellationSucrose CushionCentrifugationPBSLysis Buffer

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