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分離とクラミドモナス由来の単一粒子再構成のための蛍光イメージング
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
DOI:

10:38 min

September 21, 2018

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Chapitres

  • 00:04Titre
  • 00:46Purification of C. reinhardtii Centrioles
  • 05:15Concentration of Centrioles onto the Center of the Coverslips
  • 07:06Quantification of Isolated Centrioles on Coverslips: Centrifugation and Immunofluorescenc
  • 08:47Results: Isolation and Fluorescence Imaging of Chlamydomonas Centrioles
  • 10:03Conclusion

Summary

Traduction automatique

浄化し、多数の超解像顕微鏡法と平均単一粒子の影響を受けやすい向きが異なる由来のイメージ戦略を開発しました。

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