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SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
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Journal JoVE Biologie
SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

DOI:
10.3791/58133-v

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07:39 min

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June 28, 2019

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, , , , ,
1Department of Molecular Medicine and the Center on Aging,The Scripps Research Institute, 2Department of Biochemistry, Molecular Biology and Biophysics,University of Minnesota, 3Institute on the Biology of Aging and Metabolism,University of Minnesota

Chapitres

  • 00:04Titre
  • 01:06Generation of Senescent Murine Embryonic Fibroblast (MEF) 12-15 Days
  • 03:46Senescent Associated -Galactosidase (SA–Gal) Screening Assay 2-3 Days
  • 06:06Results: Identification of Senotherapeutics by Measuring SA–Gal Activity in Single Cells
  • 06:48Conclusion

Summary

Traduction automatique

Traduction automatique

Cellular senescence is the key factor in the development of chronic age-related pathologies. Identification of therapeutics that target senescent cells show promise for extending healthy aging. Here, we present a novel assay to screen for the identification of senotherapeutics based on measurement of senescence associated β-Galactosidase activity in single cells.

Tags

SA-β-GalactosidaseSenotherapeutic DrugsSenolyticSenomorphicHigh-throughput Drug ScreeningAging-related DiseasesCell SenescenceCell CultureTrypsinization

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