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SA-β-加拉基沙酶筛查检测,用于识别治疗药物

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Biologie

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Journal JoVE Biologie

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

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SA-β-加拉基沙酶筛查检测,用于识别治疗药物

DOI:

10.3791/58133-v

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07:39 min

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June 28, 2019

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Heike Fuhrmann-Stroissnigg*¹, Fernando E. Santiago*^1,2,3, Diego Grassi, YuanYuan Ling, Laura J. Niedernhofer^2,3, Paul D. Robbins^2,3

¹Department of Molecular Medicine and the Center on Aging,The Scripps Research Institute, ²Department of Biochemistry, Molecular Biology and Biophysics,University of Minnesota, ³Institute on the Biology of Aging and Metabolism,University of Minnesota

Chapitres

00:04Titre
01:06Generation of Senescent Murine Embryonic Fibroblast (MEF) 12-15 Days
03:46Senescent Associated -Galactosidase (SA–Gal) Screening Assay 2-3 Days
06:06Results: Identification of Senotherapeutics by Measuring SA–Gal Activity in Single Cells
06:48Conclusion

Summary

Traduction automatique

细胞衰老是慢性年龄相关疾病发展的关键因素。针对衰老细胞的治疗药物的鉴定为延长健康衰老提供了希望。在这里,我们提出了一个新的测定,以筛选基于测量与衰老相关的β-加拉托西沙酶在单细胞中的异质疗法的识别。

Tags

SA-Î²-Galactosidase Senotherapeutic Drugs Senolytic Senomorphic High-throughput Drug Screening Aging-related Diseases Cell Senescence Cell Culture Trypsinization

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