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Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells
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Journal JoVE Bio-ingénierie
Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

DOI:
10.3791/58176-v

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08:35 min

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August 24, 2018

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, , ,
1Department of Bioengineering,University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute,University of California, Los Angeles

Chapitres

  • 00:04Titre
  • 00:31Hydrogel Crosslinking and Cell Encapsulation
  • 03:11Hydrogel Culture Single Cell Extraction for Flow Cytometry and Cryopreservation for Sectioning
  • 06:10Results: Hyaluronic Acid-Based 3D Patient-Derived Glioblastoma Cell Hydrogel Cultures
  • 07:55Conclusion

Summary

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Traduction automatique

Here, we present a protocol for three-dimensional culture of patient-derived glioblastoma cells within orthogonally tunable biomaterials designed to mimic the brain matrix. This approach provides an in vitro, experimental platform that maintains many characteristics of in vivo glioblastoma cells typically lost in culture.

Tags

Hyaluronic AcidHydrogels3D Cell CultureGlioblastomaExtracellular MatrixPatient-derived CellsCell EncapsulationPEG-MalSilicone Rubber MoldsCell DissociationCell SuspensionGelation

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