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Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
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Journal JoVE Biologie
Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

DOI:
10.3791/58192-v

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07:38 min

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September 30, 2018

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, , ,
1Department of Chemistry,Universidad Autónoma de Querétaro, 2Department of Biochemical Engineering,Instituto Tecnológico de Morelia, 3Department of Biochemical Engineering,Instituto Tecnológico Superior de Ciudad Hidalgo

Chapitres

  • 00:04Titre
  • 00:31Inoculum Preparation and Growth Curves
  • 03:09Data Processing and Kinetic Parameter Calculation
  • 05:41Results: Representative Exponential Growth Kinetic Analysis
  • 07:10Conclusion

Summary

Traduction automatique

Traduction automatique

Here we present a protocol to estimate the respiratory and fermentative metabolism by fitting the exponential growth of Saccharomyces cerevisiae to the exponential growth equation. Calculation of the kinetic parameters allows for the screening of influences of substances/compounds on fermentation or mitochondrial respiration.

Tags

Saccharomyces CerevisiaeExponential Growth KineticsBatch CultureRespiratory MetabolismFermentative MetabolismWarburg EffectCrabtree EffectYeast Extract Peptone Dextrose (YPD) BrothGlycerol PreservationAgar PlateMicroplate Growth CurveShaken Conical FlaskOptical DensityAgitation Speed

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