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Recherche en cancérologie
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Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

DOI:
10.3791/58194-v

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10:09 min

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January 07, 2019

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, ,
1Cancer Blood Disease Institute, Divisions of Experimental Hematology and Cancer Pathology,Cincinnati Children’s Hospital Medical Center

Chapitres

  • 00:04Titre
  • 00:48Isolation of c-Kit+ Cells from Bone Marrow
  • 03:52Transduction
  • 06:43Colony-forming Unit Assays
  • 08:25Results: c-Fos and Dusp1 Therapeutic Target in Leukemia
  • 09:36Conclusion

Summary

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Traduction automatique

Here, we describe protocols for the genetic and chemical validation of c-Fos and Dusp1 as a drug target in leukemia using in vitro and in vivo genetic and humanized mouse models. This method can be applied to any target for genetic validation and therapeutic development.

Tags

C-FosDusp1Oncogene DependenceCancer TherapeuticsLeukemiaSolid TumorsLung CancerBrain TumorsPancreatic TumorsBone MarrowMouseCell IsolationCD117MACSCell SeparationCell Culture

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