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Micropuncture Bowmans plass i mus tilrettelagt av 2 Foton mikroskopi
Journal JoVE
Biologie
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Journal JoVE Biologie
Micropuncture of Bowman’s Space in Mice Facilitated by 2 Photon Microscopy

Micropuncture Bowmans plass i mus tilrettelagt av 2 Foton mikroskopi

DOI:
10.3791/58206-v

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07:37 min

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October 11, 2018

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, , , , , ,
1Anesthesiology & Perioperative Medicine,Oregon Health & Science University, 2Environmental and Biological Services Division,Pacific Northwest National Laboratory, 3Operative Care Division,Portland Veterans Affairs Medical Center

Chapitres

  • 00:04Titre
  • 00:42Kidney Stabilization and Injection
  • 02:35Glomerulus Selection and Bowman s Space Fluid Aspiration
  • 05:41Results: Representative Bowman s Space Micropuncture and Analysis
  • 07:11Conclusion

Summary

Traduction automatique

Traduction automatique

Vi presenterer bruk av 2-fotonet mikroskopi å plassere brønnene i Bowmans urin plass i mus, kombinere 2 grunnleggende teknikker av nyre fysiologi. Bruk av 2-fotonet mikroskopi overvinner kritiske begrensninger av konvensjonelle mikroskopi for micropuncture nyre fysiologi studier.

Tags

MicropunctureBowman’s SpaceTwo-photon MicroscopySingle-nephron PhysiologyGlomerular FiltrationTubular PhysiologyMouse PreparationKidney StabilizationFITC-DextranTarget GlomerulusPipette Positioning

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