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1Department of Experimental Embryology, Institute of Genetics and Animal Breeding,Polish Academy of Sciences, 2Department of Regenerative Medicine,Maria Skłodowska-Curie Institute – Oncology Center, 3Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding,Polish Academy of Sciences
Chapitres
- 00:04Titre
- 00:27Culture Maintenance and Sub-culturing Cells
- 02:13Trypsin Digestion
- 03:29Aggregate Generation
- 04:34Aggregate Dissociation
- 05:47Plating Cells
- 06:31Results: Neuronal Differentiation of P19 Cell Line
- 07:23Conclusion
Den P19 musen embryonale kreftcelle linje (P19 cellelinje) er mye brukt for å studere den molekylære mekanismen av neurogenesis med stor forenkling i forhold til in vivo analyse. Her presenterer vi en protokoll for retinoic acid-indusert neurogenesis i P19 cellelinjen.
