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Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
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Chapitres
- 00:04Titre
- 00:41Intrahepatic Hematopoietic Stem Cell (HSC) Injection
- 01:59HSC Engraftment Validation
- 03:48HIV Infection Analysis and Viral Suppression and Rebound Validation
- 05:20Results: Engraftment Validation and HIV Infection and Suppression Analyses
- 06:26Conclusion
This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.
