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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

DOI:
10.3791/58270-v

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09:52 min

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December 04, 2018

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, , , , , , , , ,
1Dept. of Hematology, Oncology and Immunology,University of Tübingen, 2Dept. of Endocrinology, Diabetology, Clinical Pathology and Metabolism,University of Tübingen

Chapitres

  • 00:04Titre
  • 00:41Fixation, Cell Lysis, and Chromatin Shearing
  • 03:54Chromatin Immunoprecipitation
  • 08:31Results: qRT-PCR with Precipitated DNA Identified Specific Binding of NFAT2 to the LCK Promotor Region
  • 09:30Conclusion

Summary

Traduction automatique

Traduction automatique

Chronic lymphocytic leukemia (CLL) is the most common leukemia in the western world. NFAT transcription factors are important regulators of development and activation in numerous cell types. Here, we present a protocol for the use of chromatin immunoprecipitation (ChIP) in human CLL cells to identify novel target genes of NFAT2.

Tags

Chromatin ImmunoprecipitationNFAT2Chronic Lymphocytic LeukemiaTranscription FactorsTarget GenesFixationShearingCell LysisSonicationProtease Inhibitor

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