Journal
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Biologie du développement
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Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

DOI:
10.3791/58274-v

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07:50 min

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August 29, 2018

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, , ,
1BioTime, Inc

Chapitres

  • 00:04Titre
  • 00:59Tissue Preparation for Immunostaining
  • 03:02Immunostaining with 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) Antibodies
  • 05:02Results: Immunostaining with 5mC and 5hmC to Determine Their Distribution During Retinal Development
  • 06:49Conclusion

Summary

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The objective of this report is to describe the protocol for robust immunohistochemical detection of epigenetic markers, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in developing and postmitotic mouse retina.

Tags

Immunohistochemistry5-methylcytosine5-hydroxymethylcytosineMouse RetinaRetinal DevelopmentChromatinFrozen Histological PreparationCryosectioningImmunostainingDNA Methylation

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