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Immunohistochemical påvisning av 5-Methylcytosine og 5-Hydroxymethylcytosine i utviklingen og Postmitotic musen netthinnen
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
DOI:

07:50 min

August 29, 2018

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Chapitres

  • 00:04Titre
  • 00:59Tissue Preparation for Immunostaining
  • 03:02Immunostaining with 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) Antibodies
  • 05:02Results: Immunostaining with 5mC and 5hmC to Determine Their Distribution During Retinal Development
  • 06:49Conclusion

Summary

Traduction automatique

Målet med denne rapporten er å beskrive protokollen for robust immunohistochemical påvisning av epigenetic markører, 5-methylcytosine (5mC) og 5-hydroxymethylcytosine (5hmC) i utviklingen og postmitotic musen netthinnen.

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