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Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

DOI:
10.3791/58278-v

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06:36 min

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May 01, 2019

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, , , , ,
1Department of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University, 2Hunan ClinicaI Research Center in Gynecologic Cancer, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University

Chapitres

  • 00:04Titre
  • 01:15Deparaffinization and Rehydration of All Slides
  • 02:00Staining
  • 04:04Dehydration and Mounting
  • 04:48Results: Analysis of Elastic Staining on Paraffin-embedded Slides
  • 06:03Conclusion

Summary

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Here, we present a protocol of elastic staining to identify elastic fibers in pT3N0M0 gastric cancer tissues on formalin-fixed, paraffin-embedded sections. Subsequently, we describe a method to determine whether tumor cells invade beyond the elastic lamina.

Tags

Elastic LaminaPT3 Gastric CancerTumor Cell InvasionElastic StainingParaffin-embedded SlidesPathologic StagingLung CancerColorectal CancerTNM StagingXyleneEthanolPotassium PermanganateOxalic AcidElastin StainingVan Gieson Counterstain

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