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Effektiv og skalerbar rettet differensiering av klinisk kompatibel hornhinnen Limbal epitel stamceller fra menneskelige Pluripotent stamceller
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
DOI:

10:07 min

October 24, 2018

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Chapitres

  • 00:04Titre
  • 00:41Step I: Corneal Induction in Suspension Culture
  • 03:07Step II: Corneal Differentiation in Adherent Culture
  • 04:33Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 06:08Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 07:25Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells
  • 09:31Conclusion

Summary

Traduction automatique

Denne protokollen introduserer en enkel to-trinns metode for å skille hornhinnen limbal epitel stamceller fra menneskelige pluripotent stamceller under xeno- og mater celle-fri kultur forhold. Metodene for celle-kultur presenteres her aktivere kostnadseffektive, storskala produksjon av klinisk kvalitet celler gjelder hornhinnen cellen terapi bruk.

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