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Endre Baculovirus uttrykk vektorer å produsere utskilles anlegget proteiner i insekt celler
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
DOI:

09:14 min

August 20, 2018

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Chapitres

  • 00:04Titre
  • 00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
  • 02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
  • 07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
  • 07:57Conclusion

Summary

Traduction automatique

Her presenterer vi protokollene for utnytte insekt celler og baculovirus protein uttrykk systemet å produsere store mengder anlegget utskilles proteiner for protein krystallisering. En baculovirus uttrykk vektor er endret med GP67 eller insekt hemolin signal peptid plante protein sekresjon uttrykk i insekt celler.

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