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Generation af menneskelige primordiale kønsceller-lignende celler på overfladen af Embryoid organer fra primet pluripotency induceret pluripotente stamceller
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
DOI:

12:06 min

January 11, 2019

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Chapitres

  • 00:04Titre
  • 01:01Inoculation and Conversion of Primed Pluripotency Human Induced Pluripotent Stem Cells (hiPSCs)
  • 03:40Embryoid Body (EB) Formation Using Microwell Plates
  • 05:52Transfer of EBs to Low Attachment Plates and Generation of hPGCLCs on the Surface of EBs
  • 08:00Immunohistochemical Staining of EBs
  • 09:41Results: Successful Generation of EBs with hPGCLCs on their Surface
  • 11:29Conclusion

Summary

Traduction automatique

Primordiale kønsceller (PGCs) er fælles forløbere for både sæd og æg. Menneskelige embryonale PGCs er angivet fra pluripotente epiblast celler gennem interaktioner af cytokiner. Her beskriver vi en 13-dag protokol af inducerende menneskeceller transcriptomally ligner PGCs på overfladen af embryoid organer fra PRIMES-pluripotency induceret pluripotente stamceller.

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