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1Neurobiology Laboratory,National Institute of Environmental Health Sciences, 2Comparative Medicine Branch,National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory,National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory,National Institute of Environmental Health Sciences
Chapitres
- 00:04Titre
- 00:55Mouse Fertilized Egg Isolation
- 02:12Mouse Fertilized Eggs XYClone Laser Perforation
- 03:13Mouse Fertilized Egg Transduction and Non-surgical Transfer
- 04:00Results: Representative Lentivirus Transduction Analyses
- 04:55Conclusion
Mus befruktede egg og tidlig stadium embryo er beskyttet av zona pellucida, en glykoprotein matrise som danner en barriere mot gen levering. Denne artikkelen beskriver en protokoll for perforating i zona med en laser transduce embryonale celler med lentiviral vektorer og opprette transgene mus.
