Journal
/
Environnement
/
Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation
Journal JoVE
Environnement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Environnement
Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

DOI:
10.3791/58332-v

•

10:16 min

•

October 12, 2018

•

, , ,
1Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology,Johns Hopkins University School of Medicine, 2Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology, Ophthalmology, Oncology, Neurosurgery, Materials Science and Engineering, Chemical and Biomolecular Engineering, and the Bloomberg-Kimmel Institute for Cancer Immunotherapy,Johns Hopkins University School of Medicine

Chapitres

  • 00:04Titre
  • 00:49Tunable Spherical Poly(Lactic-Co-Glycolic Acid) (PLGA) Fabrication
  • 03:30Tunable Polymeric Particle Fabrication
  • 06:21Surface Protein Conjugation for Artificial Antigen Presenting Cell (aAPC) Creation
  • 08:01Results: Representative Spherical and Ellipsoidal Particle Characterization
  • 09:49Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol to quickly and reproducibly generate biologically inspired, biodegradable articifical antigen presenting cells (aAPC) with tunable size, shape, and surface protein presentation for T cell expansion ex vivo or in vivo.

Tags

Anisotropic Polymeric Artificial Antigen Presenting CellsCD8+ T Cell ActivationParticle Physical PropertiesBiomaterial Immune Cell InteractionsBiomimetic ParticlesCancer ImmunotherapyInfectious DiseaseType 1 DiabetesPLGAPolyvinyl AlcoholMicro-particle SynthesisNano-particle Synthesis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil

Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Use of Chironomidae (Diptera) Surface-Floating Pupal Exuviae as a Rapid Bioassessment Protocol for Water Bodies

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge

Functionalization and Dispersion of Carbon Nanomaterials Using an Environmentally Friendly Ultrasonicated Ozonolysis Process

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

A Strain Gauge Monitor (SGM) for Continuous Valve Gape Measurements in Bivalve Molluscs in Response to Laboratory Induced Diel-cycling Hypoxia and pH

Bioindication Testing of Stream Environment Suitability for Young Freshwater Pearl Mussels Using In Situ Exposure Methods

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Read Article